Pierce ecl western blotting substrate plus#

Pierce ecl western blotting substrate plus#

Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blot analysis.

Higher sensitivity - detect targets down to the low-picogram level. The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. PI32106 Pierce ECL Western Blotting Substrate 500mL kit PI80197 Pierce ECL 2 Western Blotting Substrate Sufficient reagent for 250cm2 of membrane Detection Reagent A: 25mL Detection Reagent B: 0. Easy to use, high quality and performance. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. The Pierce ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP, which produces a strong chemiluminescent signal in the presence of peroxide that can be captured by X-ray film CCD imaging.

LC-MS innovation: Improve analytical accuracy English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian esk. Finally, the membranes were developed with Pierce ECL Western Blotting Substrate (Thermo Fisher. PierceÂ® ECL Plus Western Blotting Substrate. Which microbioreactor modules are best for you (Jackson ImmunoResearch, West Grove, PA, United States). Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.Which solid support is right for your oligo synthesis? This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or an imaging system. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.īecause the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate enables the detection of picogram amounts of antigen. Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity.

Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)Ĭompare and learn more about our western blot chemiluminescent substrates ›.

Compatibility: nitrocellulose and PVDF membranes.

Thermo Scientific Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blot and IHC experiments. Bioz Stars score: 86/100, based on 1 PubMed citations. 1-Step TMB-Blotting Substrate Solution Thermo Scientific.

Stability: 1-hr working solution stability, 1-year kit stability at 4☌ Thermo Fisher pierce ecl western blotting substrate enhanced chemiluminescence system Pierce Ecl Western Blotting Substrate Enhanced Chemiluminescence System, supplied by Thermo Fisher, used in various techniques.

Pierce ECL Plus Western Blotting Substrate characteristics include: (3) Pierce ECL Western Blotting Substrate For Clear Observation Of Bands Ab G The Pierce ECL Western Blotting Substrate is a simple set of reagents to allow quick and easy observation of western blotting. The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots. Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. Very Good Western Blotting Substrate from Thermo Xuewei Wang Our lab will keep using this kit in western blot experiments.